Fig. 7: HIF-1α is crucial for the therapeutic effect of LGG-s in nicotine-exposed MASH mice.
From: Nicotine exacerbates MASH via inducing intestinal dysbiosis and barrier dysfunction
a H&E staining of the intestine and liver in mice. Scale bar = 200 μm. b, c Serum ALT and AST levels in mice (n = 6). d–g Intestinal gene expression of pro-inflammatory cytokines Il1b, Il6, and Tnf-α, and anti-inflammatory cytokines Il10 (n = 6). h–j Gene expression of ZO-1, occludin, and claudin-1 (n = 6). k–n Western blot analysis for ZO-1, occludin, and claudin-1 normalized by β-actin (n = 3). o The representative images of the structure of the intestinal barrier of mice. Arrows point to cell-cell junction under TEM. a Tight junction; b adherens junction; c desmosome; d gap junction. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Data are presented as mean ± SD.
