Fig. 7: KDM6A loss of function induces the promoter regions accessibility changes of CDH2, CDH3, and CDH12 to promote PANC-1 migration and invasion. | Communications Biology

Fig. 7: KDM6A loss of function induces the promoter regions accessibility changes of CDH2, CDH3, and CDH12 to promote PANC-1 migration and invasion.

From: Multi-omics elucidation of KDM5C, KDM6A, and KMT2B roles in cancer epigenetic dysregulation and transcriptional reprogramming

Fig. 7

a Integrative genomic viewer (IGV) snapshot of accessible chromatin peaks at CDH2, CDH3, and CDH12 gene promoter regions in KDM6AKO and WT cells. b Relative expression of CDH2, CDH3, and CDH12 genes from RNA-seq data. Relative expression of CDH2, CDH3, and CDH12 genes was assessed in c HEK293T-KDM6AKO and d PANC-1-shKDM6A cells by real-time fluorescent quantitative PCR (RT–qPCR). e, f Representative images and quantifications of migration capability of control and PANC-1-shKDM6A cells by wound healing assay. Bar: 200 μm. g, h Representative images and quantifications of invasion capability of control and PANC-1-shKDM6A cells by transwell assay. Bar: 100 μm. ****p < 0.0001.

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