Fig. 5: Dnmt3a2 loss reduces the methylation of infertility-related imprinted genes in mouse sperm cells.
From: Dnmt3a2 expression during embryonic development is required for phenotypic stability
A DNA methylation violin plot showing the distribution of the β-values of the autosomal probes in the Infinium mouse EPIC array. β-values range from 0.0 to 1.0 for each probe, indicating total lack of methylation or complete methylation, respectively. The right panel compares the median β-value for the WT to the Dnmt3a2−/− sperm. Biological replicates are represented by individual dots. ****, p < 0.0001 by two-tailed unpaired t-test. B Heatmap showing hypomethylated CpG sites in Dnmt3a2−/− mice compared to WT. Hypomethylated probes were selected using a cutoff of β-value difference greater than 0.2 and p < 0.05 by two-tailed unpaired t-test. Methylation levels are represented by a cold to warm color scale (β-value 0-1, 0–100% methylated), where every row represents an individual probe, and every column represents sperm from an individual mouse. C Heatmap depicting the enrichment score of hypomethylated probes (DMPs) for multiple genomic elements included in the Infinium EPIC array. The classified genomic elements are as follows: CTCF binding sites (CTCF), CpG island (CGI), transcription start site (TSS), enhancer (Enhancer), CpH methylation (CPH), repetitive elements (RMSK), gene bodies (GeneBody), partially methylated domains (PMD), differentially methylated regions (DMR), random location probes (Random), monoallelic methylation, including the ICRs of imprinted genes (MonoallelicMeth), and metastable alleles and variably methylated regions (VMRs). D Venn diagram depicting the intersection between genes from monoallelic hypomethylated DMPs identified by the EPIC array and mouse imprinted genes. E Heatmap of hypomethylated CpG sites at imprinted DMRs in sperm from fertile Dnmt3a2−/− mice. Probes were selected using a cutoff of a β-value difference > 0.2 and p < 0.05 (two-tailed unpaired t-test). Methylation levels (β-value from 0 to 1 representing 0–100% methylation) are indicated by a cold to warm color scale. Each row represents an individual probe, and each column represents an individual mouse.
