Fig. 6: Dnmt3a2 loss induces CTCF binding site loss of methylation at the H19 ICR.
From: Dnmt3a2 expression during embryonic development is required for phenotypic stability
A Schematic representation of the H19 5’ Upstream region. The studied regions of interest (ROI 1 and ROI 2, blue box) are located inside the ICR (Green box) encompassing two CTCF binding sites (magenta boxes), and at the promoter region of H19, respectively. Bar graphs depicting the percentage of unmethylated CpGs in sperm DNA from WT and Dnmt3a2−/− mice at the following loci: the H19 ICR CTCF binding sites (ROI 1) (B), the H19 promoter (ROI 2) (C), the Snrpn DMR and enhancer (D), and the ICR of Grb10 (E). Schematic representation of the Snrpn and Grb10 genes are shown in the top (D, E), where exons are depicted as blue boxes. The ICR/DMR is represented by a green box and the enhancer by a yellow box. The region of interest (ROI) amplified for targeted amplicon bisulfite sequencing is shown, with individual CpG sites (black lollipops) and their corresponding numbers indicated. Biological replicates are represented by individual dots. Data are presented as mean ± SEM. Significance was determined by two-tailed unpaired *t*-tests (*, p < 0.05).
