Fig. 6: Immune cells isolated from the lymph node (LN) of AireC313X−/− and WT mice display similar interferon signature across genotypes.
From: Aire-dependent interferon signalling shapes thymocyte maturation and central tolerance in mice
a UMAP plots of LN immune cells in AireC313X−/−and WT mice. b Relative abundance of cells in LN clusters across AireC313X−/− UMAP and WT mice, n = 4 per genotype. UMAP visualisation and distribution of interferon (IFN) module scores in LN immune cells in AireC313X−/− and WT mice for c, d ‘response to type I interferon’, e, f ‘response to type II interferon’, or g, h thymocyte ISG DEGs. i Heatmap with log2fc values of differentially expressed genes (DEGs) in the LN dataset. Colour scale red: upregulated and blue: downregulated genes in AireC313X−/− mice. Gene names coloured red are DEGs corresponding to enriched GO terms ‘response to virus’. For UMAP plots in (c, e, g), expression of features in AireC313X−/− and WT mice are shown on a common colour scale. For boxplots in (c, e, g), clusters are ordered by increasing median values for WT, for n please see Supplementary Data 4.9.
