Table 1 Primers used for qPCR
From: Aire-dependent interferon signalling shapes thymocyte maturation and central tolerance in mice
Gene | Forward (5′ –> 3′) | Reverse (5′ –> 3′) |
|---|---|---|
Actb | GGCTGTATTCCCCTCCATCG | CCAGTTGGTAACAATGCCATGT |
Gadph | AGGTCGGTGTGAACGGATTTG | TGTAGACCATGTAGTTGAGGTCA |
Hprt | TCAGTCAACGGGGGACATAAA | GGGGCTGTACTGCTTAACCAG |
Ifi27l2a | GAACACTGTTTGGCTCTG | CTGCTGATTGGAGTGTGGCTAC |
Irf7 | GAGACTGGCTATTGGGGGAG | GACCGAAATGCTTCCAGGG |
Isg15 | GGTGTCCGTGACTAACTCCAT | TGGAAAGGGTAAGACCGTCCT |
Ly6a | AGGAGGCAGCAGTTATTGTGG | CGTTGACCTTAGTACCCAGGA |
Rtp4 | TGGGAGCAGACATTTCAAGAAC | ACCTGAGCAGAGGTCCAACTT |
Stat1 | TCACAGTGGTTCGAGCTTCAG | GCAAACGAGACATCATAGGCA |
Usp18 | TTGGGCTCCTGAGGAAACC | CGATGTTGTGTAAACCAACCAGA |
