Fig. 5: Outer hair cell death in Klhdc7bRegnΔ/Δ mice.
A Immunostaining of whole mounted cochlea of Klhdc7bRegn+/+, and Klhdc7bRegnΔ/Δ mice at postnatal day (p) 6, p11, 3 weeks and 8 weeks of age. MYO7A (green) specifically labels hair cells, DAPI (blue) stains nuclei, F-actin (white) labels stereocilia. Arrows denote missing OHCs. Arrowheads denote examples of supporting cell scars where OHCs have died. Asterisks denote rounded MYO7A staining of cells outside the normal hair cell location. B Cochleae were stained for MYO7A and a nuclear stain, then cleared and imaged using light-sheet microscopy. For one example cochlea, Left, MYO7A staining in 3D. Middle, automatically generated outer hair cell mask. Right, frequency mapping along the tonotopic axis from apex to base. C Representative example images of Klhdc7bRegn+/Δ (left) and Klhdc7bRegnΔ/Δ (right) cochleae at five weeks visualized from the top down. Below each image of intact cochlea are example images of apex (left) and middle (right) turns. D Outer to inner hair cell ratios at three weeks and five weeks from Klhdc7bRegnΔ/+ and Klhdc7bRegnΔ/Δcochleae. n = 3 mice per genotype, two-tailed Welch’s t-test, *p < 0.05, all error bars show standard deviation.
