Fig. 6: Progressive outer hair cell loss in Klhdc7bIMPC-/- mice is evident from 3 weeks of age. | Communications Biology

Fig. 6: Progressive outer hair cell loss in Klhdc7bIMPC-/- mice is evident from 3 weeks of age.

From: Cross-species validation of a human age-related hearing loss candidate KLHDC7B as essential for mammalian hearing

Fig. 6: Progressive outer hair cell loss in Klhdc7bIMPC-/- mice is evident from 3 weeks of age.The alternative text for this image may have been generated using AI.

A–C Immunofluorescence in wholemount cochlea preparations using MYO7A to label hair cells in Klhdc7bIMPC+/+, Klhdc7bIMPC+/- and Klhdc7bIMPC-/- mice. Panels show (x20) representative confocal microscopy images from all three genotypes. Charts to the right show OHC quantification at each cochlea turn, base, mid and apical. Scale bar = 5 µm. Data is shown for 3 ages: A postnatal day 2–4; B 3 weeks and at 8 weeks of age (C). Arrowheads show examples of missing outer hair cells. Sex of animals in images (A) was not assigned in neonatal animals. B From left to right, base: female/male/female; mid: female/ male/ female; apex: female/male/female. C From left to right, base: female/female/male; mid: male/ male/ male; apex: female/male/male. D Chart shows quantification of inner hair cells (IHCs) at 8 weeks of age. Statistical analysis was performed via one-way ANOVA, using Tukey’s test for post-hoc comparisons, ns not significant, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, all error bars show standard deviation.

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