Fig. 2: Pipp ablation promotes mammary ductal multi-layering in Pten+/− mice. | Communications Biology

Fig. 2: Pipp ablation promotes mammary ductal multi-layering in Pten+/− mice.

From: Non-redundant roles of the phosphoinositide phosphatases PTEN and PIPP in PI3K/AKT signaling in breast cancer

Fig. 2: Pipp ablation promotes mammary ductal multi-layering in Pten+/− mice.

A FFPE sections of mammary glands from 16 w-old wild-type, Pipp−/−, Pten+/− and Pipp−/−;Pten+/− mice were stained with H&E. Higher magnification images of the boxed regions are shown. B–E FFPE sections of mammary glands from 150 d (B) and 210 d (D) Pten+/− and Pipp−/−;Pten+/− mice were immunostained with a cytokeratin 8 antibody. Arrow in (D) indicates intraductal luminal epithelial cells in the luminal space. Data represent mean percentage of multilayered ducts ± SEM from 150 d (C) (Pten+/− n = 7 and Pipp−/−;Pten+/− n = 8) and 210 d (E) (Pten+/− n = 10 and Pipp−/−;Pten+/− n = 12) mice. F, G Mammary gland whole mounts from wild-type, Pipp−/−, Pten+/− and Pipp−/−;Pten+/− mice at 150 days of age were stained with Carmine alum (F). The data represent the mean number of branch points ± SEM from 150 d (wild-type n = 10, Pipp−/− n = 9, Pten+/− n = 9 and Pipp−/−;Pten+/− n = 7) (G) mice. Scale bars, 50 μm (A, 25 μm in higher magnification images), 100 μm (B, D), 500 μm (F).

Back to article page