Fig. 6: Co-shRNA knockdown of PIPP and PTEN enhances AKT signaling. | Communications Biology

Fig. 6: Co-shRNA knockdown of PIPP and PTEN enhances AKT signaling.

From: Non-redundant roles of the phosphoinositide phosphatases PTEN and PIPP in PI3K/AKT signaling in breast cancer

Fig. 6: Co-shRNA knockdown of PIPP and PTEN enhances AKT signaling.

A–E MDA-MB-231 PIPP, PTEN, PIPP/PTEN or control shRNA cells were serum starved overnight, stimulated with 100 ng/ml EGF for the indicated times then lysed and immunoblotted with pAKT Thr308, pAKT Ser473, pAKT1 Ser473, pAKT2 Ser474, AKT or GAPDH antibodies (A). Data represent mean pAKT Thr308 (n = 4) (B), pAKT1 Ser473 (n = 4) (C) or pAKT2 Ser474 (n = 6) (D) relative to AKT ± SEM or AKT relative to GAPDH (n = 3 independent experiments) (E) ± SEM. F, G MDA-MB-231 PIPP, PTEN, PIPP/PTEN or control shRNA cells were serum starved overnight, stimulated with 100 ng/ml EGF for the indicated times, then lysed and immunoblotted with pPRAS40 Thr246, PRAS40 or GAPDH antibodies (F). Data represent mean pPRAS40 Thr246 relative to PRAS40 (n = 5 independent experiments) (G) ± SEM.

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