Table 2 Isolation of stable overexpression transgenic parasites under drug selection
Passage No. | Gene ID | No. of birds | Age (d) | Dose of oocysts/sporocysts given | Drug selection | Concentration (mg/kg) | Time of oocysts collection (d) | Fluorescence rate (%) |
|---|---|---|---|---|---|---|---|---|
1 | ETH2_0402100 | 5 | 23 | 5 × 103 | Maduramicin | 5 | 6–9 | 0.3 |
ETH2_0402400 | 5 | 23 | 5 × 103 | 0.2 | ||||
ETH2_0402600 | 5 | 23 | 2 × 103 | 0.3 | ||||
2 | ETH2_0402100 | 5 | 23 | 5 × 103 | Maduramicin | 5 | 6–12 | 21.0 |
ETH2_0402400 | 5 | 23 | 5 × 103 | 0 | ||||
ETH2_0402600 | 5 | 23 | 2 × 103 | 0 | ||||
3 | ETH2_0402100 | 3 | 33 | 6 × 103 | Maduramicin | 5 | 6–9 | 40.0 |
4 | ETH2_0402100 | 6 | 7 | 2 × 105 (sporocysts) | Maduramicin | 5 | 6–9 | 62.0 |
5 | ETH2_0402100 | 6 | 20 | 3 × 106 (sporocysts) | Maduramicin | 5 | 6–9 | 85.0 |
6 | ETH2_0402100 | 6 | 15 | 2 × 106 (sporocysts) | Maduramicin | 5 | 6–9 | 98.0 |
