Fig. 3: Propofol promotes p16INK4a expression to induce hippocampal neuronal senescence.
A Representative SA-β-gal staining in the indicated HT22 cells. B, C Quantification of SA-β-gal positive cells in the indicated HT22 cells. D Expression of H3K27me3, H3K9me3 and HP1γ were determined by Western blotting analysis in HT22 cells treated with propofol or saline (Control). E The relative expression of H3K27me3, H3K9me3 and HP1γ by normalizing against β-actin expression. Immunostaining HIRA and PML (F, G) and macroH2A1.2 H, I in the indicated HT22 cells and hippocampal-derived primary neurons. J, K Representative SA-β-gal staining and quantification of SA-β-gal positive cells in the hippocampus of indicated group. L A Schematic representation of viral injections into the hippocampus and representative IHC images of DAPI (blue) and GFP (green, viral marker) 6 weeks after viral injections. Scale bars represent 250 µm. M Escape latencies (s) of indicated groups in the MWM test. N TNF-α, IL-1β and IL-6 levels were detected in the hippocampus of indicated group. For all figures: Values mean ± SD. Statistical significance was assessed using two-way ANOVA with post hoc Tukey’s multiple comparisons test. *P < 0.05, **P < 0.01, and ***P < 0.001.
