Fig. 3: PLys is a potent lysozyme under acidic conditions.
From: An ancient lysozyme in placozoans participates in acidic extracellular digestion
A SDS-PAGE gel of purified recombinant mPLys_M112 and pPLys. The molecular mass standard is indicated on the left and marked with the associated molecular masses in kDa. B Lysozyme assay of 32 pmol pPLys, 32 pmol mPLys_M112, and 32 pmol HEWL at pH 5.0. Reaction buffer consisted of 20 mM sodium acetate, pH 5.0 (I = 13 mM). Dependency of lysozyme activity of pPLys and mPLys_M112 on ionic strength (C) and pH (D) at room temperature. E Lysozyme activity of pPLys, mPLys_M112 and HEWL at different temperatures. All measurements were performed in triplicates. Bacterial lysis assay against B. subtilis (F) and E. coli D31 (G) using a membrane-impermeable dye (SYTOX Green). Fluorescence indicates lysis of cells. Sigmoidal functions were fitted to the dose response curves. mPLys_M112: open triangles, pPLys closed triangles, HEWL closed squares, Melittin closed circles, buffer control: crosses.
