Fig. 7: Effect of the stressors on cellular accumulation of neutral lipids.

A Results of the stressors impact analysis for cellular lipid content in WT VSMC, KO VSMC and EC. B Exemplary microscopy images of VSMC and EC stained with LipidTox under control conditions and after exposure to all stressors. C Results of the stressors impact analysis for subcellular lipid content in WT VSMC, KO VSMC and EC. Spot-like lipid accumulation in the cell periphery (yellow arrow) and perinuclear accumulation were analyzed separately. N = 6 plates with 6 wells for each condition and each cell type. D Increased cellular lipid content resulted from triglyceride accumulation. N = 6. Error bars represent 95% confidence intervals.