Fig. 3: Automated 3D skeletal tracking for motion trajectory analysis. | Communications Biology

Fig. 3: Automated 3D skeletal tracking for motion trajectory analysis.

From: High-throughput multi-camera array microscope platform for automated 3D behavioral analysis of swimming zebrafish larvae

Fig. 3: Automated 3D skeletal tracking for motion trajectory analysis.

a Workflow of the 3D skeletal tracking pipeline, including image pre-processing and key point extraction of zebrafish larvae using machine learning algorithms. The extracted key points form the skeleton in top and side views and are then combined to reconstruct 3D trajectories. Color bar: time. b,c Skeletal tracking from top and side views at several time points, fused 3D skeletons, and 3D motion trajectories of confined zebrafish larvae (bottom left), and 2D projections along the x − y and y − z planes (bottom right).

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