Fig. 6: PcNAC048 is a transcriptional inhibitor of PcPTSPro (1865bp). | Communications Biology

Fig. 6: PcNAC048 is a transcriptional inhibitor of PcPTSPro (1865bp).

From: Construction of a single-cell transcriptome atlas for Pogostemon cablin embryoids reveals PcNAC048 as a dual regulator coordinating lateral root morphogenesis and patchouli alcohol biosynthesis

Fig. 6

A Y1H assays showed that PcNAC048 interacts with PcPTSPro. The interaction between PcNAC048 and PcPTSPro was indicated by the ability of yeast cells to grow on the SD lacking His, Leu, and Trp. pHis2-p53 + pGAD53m was used as a positive control, while pHis2-PcPTSPro+pGADT7 was used as a negative control. B Schematic diagrams of the reporter and effector used in the dual-LUC (dual-luciferase) assay. REN, Renilla luciferase; PcPTSPro, the promoter of PcPTS. C Dual-LUC imaging analysis. The Agrobacterium liquid containing PcPTSpro::LUC + 35S and PcPTSpro::LUC + 35S::PcNAC048 was injected into the leaves at the distal axis of Nicotiana benthamiana, respectively. The redder the area, the stronger the fluorescence. D Relative LUC activity assay in N. benthamiana leaves. n = 3 biologically independent samples. (Student’s t-test, ****p < 0.0001).

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