Fig. 8: EE suppresses cisplatin-induced ototoxicity by the MAPK/NF-κB/NLRP3 signaling pathway. | Communications Biology

Fig. 8: EE suppresses cisplatin-induced ototoxicity by the MAPK/NF-κB/NLRP3 signaling pathway.

From: Eleutheroside E alleviates cisplatin-induced ototoxicity by down-regulating MAPK/NF-κB/NLRP3 signaling pathway and inhibiting cochlear cell pyroptosis

Fig. 8: EE suppresses cisplatin-induced ototoxicity by the MAPK/NF-κB/NLRP3 signaling pathway.

a–g The MAPK and NF-κB signaling pathway-related proteins expression in HEI-OC1 cells. Analyzing the grayscale of the corresponding proteins. The LC used was β-actin. h–n The NLRP3/Caspase-1 signaling pathway-related proteins expression in HEI-OC1 cells. Analyzing the grayscale of the corresponding proteins. The LC used was β-actin. o–t Expression of pyroptosis-related genes among each group by qRT-PCR. u, v Detection of pro-inflammatory factors in different groups using an ELISA kit. ****P < 0.0001, ***P < 0.001, **P < 0.01 and *P < 0.05 vs the Ctrl group. ####P < 0.0001, ###P < 0.001, ##P < 0.01 and #P < 0.05 vs the Cis group. Data are presented as mean ± s.e.m, n = 3 independent experiments.

Back to article page