Fig. 4: NLRP10 binds non-oxidized mitochondrial DNA. | Communications Biology

Fig. 4: NLRP10 binds non-oxidized mitochondrial DNA.

From: NLRP10 engages oxidized DNA through a Schiff-base mechanism and dissociates from NLRP3 upon inflammasome activation

Fig. 4: NLRP10 binds non-oxidized mitochondrial DNA.

A Superposition of NLRP10 (gray PDB 2M5V) with hOGG1 (rainbow PDB 8VX6) interrogating a non-oxidized guanine. B Superposition from (A) with residues within 5 Å from non-oxidized guanine shown in magenta and lysine close to the catalytic site shown in cyan. C SDS Page gel following flag column purification of NLRP10 (left). PVDF membrane probed for NLRP10 following FLAG column purification (right). D Fluorescence polarization of cy5-labeled 20 bp non-oxidized DNA incubated with increasing concentrations of NLRP10. Error bars: mean ± SEM, analyzed with one-way ANOVA. N = 3, ∗∗p = 0.0011 and 0.0072 and ∗∗∗p = 0.0003, 0.0009, and 0.0007.

Back to article page