Fig. 2: cAMP signal propagation in a chemotactic giant cell. | Communications Biology

Fig. 2: cAMP signal propagation in a chemotactic giant cell.

From: Establishing functional giant Dictyostelium cells reveals front–rear polarity in intracellular signaling

Fig. 2

a Time-lapse fluorescence images of Flamindo2-expressing giant cells after starvation for 7 h in DB showing spatial changes in cytoplasmic cAMP levels (inverted intensity). Fluorescence imaging was performed using a confocal microscope with a 40× objective. Cells were observed at 5 s intervals. The elapsed time from the start of imaging was shown. Scale bars: 20 μm. b Kymograph of cAMP signal intensity extracted from the white rectangle in (a) (46 µm in length), showing an earlier increase at the front (F) and an earlier decrease at the rear (R) over time.

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