Fig. 1: PGF2α synthesis via cell-free metabolic engineering platform. | Communications Biology

Fig. 1: PGF2α synthesis via cell-free metabolic engineering platform.

From: Improved de novo production of prostaglandin F2α from glucose with engineered Yarrowia lipolytica strains

Fig. 1: PGF2α synthesis via cell-free metabolic engineering platform.The alternative text for this image may have been generated using AI.

A Diagram of cell-free PGF2α biosynthesis. The gray schematic (created in Biorender) illustrates the biosynthetic pathway for the conversion of ARA to PGF2α. Molecular structures of ARA, PGG₂, PGH₂, and PGF2α are displayed. The PGHS catalyzes the conversion of ARA to PGH₂ utilizing heme, O₂ and NADPH. The PGFS converts PGH₂ to PGF2α with NADH. B PGF2α production via cell-free platform from ARA. n = 3. C LC-MS/MS MRM chromatogram (EIC) of PGF2α showing the quantitative transition (m/z 353 > 309.2). The standard and the synthesized PGF2α are shown in the top and bottom panels of Fig. 1C, respectively.

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