Fig. 4: Naïve B cell is characterized by a confined HP and an individualized activation pattern. | Communications Biology

Fig. 4: Naïve B cell is characterized by a confined HP and an individualized activation pattern.

From: Single-cell analysis reveals multi-faceted features of B cell development, together with age-associated B cell subpopulations

Fig. 4: Naïve B cell is characterized by a confined HP and an individualized activation pattern.The alternative text for this image may have been generated using AI.

Transcriptomic-based inference of developmental trajectory for three naïve B cell subpopulations with monocle2. Both B cell subpopulation distribution (A) and pseudotime prediction (B) are shown along the trajectory. The top 10 enriched biological processes as sorted by significance score (p-value) for FOShi (C) and HSPA1Ahi (D) naïve B cell subpopulations. A longer and lighter bar indicates a more significant score. Fisher’s exact test (two-sided, corrected), *, p < 0.05; **, p < 0.01, ***, p < 0.001. E Gene expression of a TF (HMGA1) and 6 enzyme genes (GAPDH, NDUFB2, PRDX1, PKM, SOD1 and TPI1) was highly expressed in the HSPA1Ahi subpopulation in three naïve subpopulations and the succeeding GC B cell subpopulations. F Overlapping of commonly expressed genes (CEGs) of three naïve subpopulations. G BCR isotype composition for HSPA1Ahi, LZ GC and DZ GC B cells (based on King’s dataset). BCR heavy chain variable (H) and joining (I) gene usage for the primary and FOShi naïve B cell subpopulations. For variable genes, only those with a usage frequency of at least 1% are shown. J Pie charts showing the relative frequencies of the top 100 clonotypes for 3 naïve B cell subpopulations. K Venn diagram showing clonotype sharing between primary and FOShi naïve B cell subpopulations for two donors (D2 and D3). Numbers on the diagram indicate the number of clonotypes in each compartment.

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