Fig. 3: Summary of targeted scRNA-seq methods. | Communications Biology

Fig. 3: Summary of targeted scRNA-seq methods.

From: A practical guide to targeted single-cell RNA sequencing technologies

Fig. 3: Summary of targeted scRNA-seq methods.The alternative text for this image may have been generated using AI.

RT: reverse transcription. Methods belonging to the five categories are colored accordingly. Arrows in bold indicate the main steps of the scRNA-seq protocol and are subdivided into dT priming and capture, while lighter arrows indicate additional steps which are needed for specific targeting towards TOI and ROI detection. Methods shown in the figure include CytoSeq15, tss299, Transcriptome resampling106, DART-seq94, TARGET-seq104, GoT98, BART-Seq103, BD Rhapsody targeted amplification100, Direct capture Perturb-seq96, HyPR-seq110, Constellation-Seq97, scCapture-seq107, scFAST-seq33, Method by Van Horebeek et al.102, scG2P105, RoCKseq / RoCK and ROI95, 10x Flex109, HybriSeq108.

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