Fig. 4: Summary of technologies coupling long-read sequencing with 3′/5′ based library generation. | Communications Biology

Fig. 4: Summary of technologies coupling long-read sequencing with 3′/5′ based library generation.

From: A practical guide to targeted single-cell RNA sequencing technologies

Fig. 4: Summary of technologies coupling long-read sequencing with 3′/5′ based library generation.The alternative text for this image may have been generated using AI.

The full-length library also shows fragmented transcripts which were generated by biases during early library generation steps, including reverse transcription. Methods shown in the figure include ScISOr-Seq119, RAGE-Seq118, ScNaUmi-seq120, Method by Chen et al. 126, RaCH-seq122, MAS-ISO-seq124, HIT-scISOseq123, GoT-Splice101, scTaILoR-seq125, Method by Tian et al.121.

Back to article page