Fig. 1: MPXV clade Ib spreads more efficiently than global clade IIb in cell culture. | Communications Biology

Fig. 1: MPXV clade Ib spreads more efficiently than global clade IIb in cell culture.

From: Enhanced virulence of mpox virus clade Ib over clade IIb in the CAST/EiJ mouse model

Fig. 1: MPXV clade Ib spreads more efficiently than global clade IIb in cell culture.The alternative text for this image may have been generated using AI.

To assess viral replication kinetics of MPXV clades Ib and IIb, BS-C-1 (a, d, e) or HFF-1 (b, f, g) cells were infected with 5 pfu/cell in the one step growth curves (OS, a, b) or 0.05 pfu/cell in the multi-step growth curves (MS, d–g). In every case, cell-associated virus (CAV) and extracellular virus (EV) were determined at the times post infection indicated. Triplicates were used and data shown are mean ± SD. *, p < 0.05 using an unpaired t test. c Viral plaque size quantification at 3 and 4 dpi (n = 30). ns; non-significant. h Representative images of the comet assays conducted in BSC-40 with MPXV clades IIb (up) and Ib (down).

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