Fig. 2: LSD mice have lower Oxtr mRNA expression in the IDSC.
From: Early life adversity impairs visually evoked innate defensive behaviors via oxytocin signaling
a Representative immunofluorescence images of OT neurons in the PVN and SON of control (left) and LSD mice (right) (scale bar, 100 μm). b Immunofluorescence statistics of no group differences in the number of OT neurons in LSD mice in either the PVN or SON (n = 6 for the control group; n = 6 for the LSD group). c No group difference in the total level of in vitro OT peptide in the PVN and SON of LSD mice (n = 7 for the control group; n = 7 for the LSD group). d No group difference in the total level of OT peptide in the SC of LSD mice (n = 6 for the control group; n = 6 for the LSD group). e Representative FISH image of c-fos+ cells co-stained with Oxtr in the IDSC of control (left) and LSD mice (right) (coronal sections, c-fos, green; Oxtr, red; DAPI, blue; scale bars, 500 μm and 5 μm). f FISH statistics showing decreased c-fos+ cell density in the IDSC of LSD mice (n = 6 for the control group; n = 6 for the LSD group). g FISH statistics showing decreased Oxtr+ cell density in the IDSC of LSD mice (n = 6 for the control group; n = 6 for the LSD group). h FISH statistics showing decreased intensity mean value of Oxtr in the c-fos+ cells of LSD mice (n = 360 cells from 6 mice for the control group; n = 360 cells from 6 mice for the LSD group). Student’s t test for (b–d, f, g), Mann-Whitney U-test for h; **p < 0.01, ***p < 0.001.
