Fig. 3

Effect of co-encapsulation of gelsolin on liposome deformation. a Fluorescence images showing F-actin in the bulk solution, phase contrast images showing liposomes encapsulating actin, and models for liposome deformation, in the absence or presence of gelsolin. A solution of rhodamine-phalloidin-labeled F-actin (76 μM) without or with a 1/100 molar ratio of gelsolin (760 nM) was diluted to 1 nM actin and then observed by fluorescence microscopy, or was encapsulated in liposomes as it is without dilution. Liposomes observed here were prepared with S/G. b, c Graphs show the distributions of F-actin length in the solutions and the aspect ratio of liposomes prepared with the solutions, respectively. Conditions are as shown in a. Red bars and red open circles indicate the results in the absence of gelsolin, and blue bars and blue filled circles indicate the results in the presence of gelsolin. d Phase contrast and polarization images of an individual liposome co-encapsulating F-actin and gelsolin. A solution of F-actin (70 μM) with a 1/1000 molar ratio of gelsolin (70 nM) was co-encapsulated in the liposome. All scale bars indicate 20 μm