Fig. 4

Absolute temperature measurements in ex vivo mouse tissue samples. Absolute temperature measurements in brain, kidney, liver, and muscle samples were performed using either pre-calibration of frequency shift thermal coefficient difference \(\Delta \alpha\) and intercept difference \(\Delta {\sigma }_{0}\) from 0.3% NaCl solution (fitted values), or self-calibration of \(\Delta \alpha\) and \(\Delta {\sigma }_{0}\) using 3 known temperatures (25, 35 and 45 \({}^{\circ }\)C, red dots) and blind measurements (blue dots). a Brain, pre-calibrated. b Brain, self-calibrated. c Kidney, pre-calibrated. d Kidney, self-calibrated. e Liver, pre-calibrated. f Liver, self-calibrated. g Muscle, pre-calibrated. h Muscle, self-calibrated. All temperatures are in \({}^{\circ }\)C.