Fig. 2: Screening and characterization of temperature-sensitive mutants of KaiC.

a Temperature dependence of the relationship between the ATPase activity of KaiC alone and in vitro KaiC phosphorylation-cycle (P-cycle) frequency (f_P = 24/period) in the presence of KaiA and KaiB. P-cycles of b KaiC^WT, c KaiC^S258A, d KaiC^F247A, e KaiC^Q361E, f KaiC^N390A, g KaiC^F470Y at four different temperatures: 303 (blue), 308 (green), 313 (orange), and 318 K (red). Dark-colored circles correspond to the mean from independent preparations and measurements (pale-colored squares, triangles, and diamonds). Light blue circles plotted in panel b correspond to the P-cycle of KaiC^WT in a D₂O buffer. h Correlation of Q10 values between ATPase (Q10_ATP) and P-cycle (Q10_fp). Error bars correspond to one standard deviation obtained from three independent experiments, except that they are shown as three standard deviations for Q10_fp.