Fig. 1: Schematic of three different labeling strategies.
From: MINFLUX reveals nonnormal distribution of linkage errors in immunofluorescence labeling
a Three types of antibodies labeled on a microtubule. The details of antibody structures include the nanobody (i.e., S reporter) (b), the primary Y antibody (i.e., M reporter) (c), and the secondary antibody bound to the Y antibody (L reporter) (d). b1–d1 3D representations of the structures used in the experiment. b2–d2 Diagrams of cross-sections illustrating microtubule labeling of different antibodies, with structural flexibility represented by the positions of fluorophores (red dot or red cycle in this figure), the geometric angles \(\phi\), and the rotational angles angle \(\theta\). b3–d3 Diagrams of cross-sections illustrating microtubule labeling of different antibodies without structural flexibility. “4 nm” and “12.5 nm” represent the molecular sizes for the nanobody and the Y antibody, respectively, and d is the diameter of the microtubule measured by the labeling strategy without any structural flexibility.
