Table 3 Key parameters of the MINFLUX 2D iteration sequence
From: MINFLUX reveals nonnormal distribution of linkage errors in immunofluorescence labeling
2D imaging | Center frequency ratio limits (CFR) | Dwell time (ms) | L-size (nm) | Minimal photon count | Laser power factor | TCP | Pattern repeat | Background threshold (kHz) |
|---|---|---|---|---|---|---|---|---|
Pre-scan | Off | \(\ge\)1 | 288 | 160 | 1 | Hexagon | 1 | 15 |
1st iteration | 0.5 | \(\ge\)1 | 288 | 150 | 1 | Hexagon | 5 | 10 |
2nd iteration | Off | \(\ge\)1 | 151.2 | 100 | 2 | Hexagon | 5 | 10 |
3rd iteration | 0.8 | \(\ge\)1 | 75.6 | 100 | 4 | Hexagon | 5 | 10 |
4th iteration | Off | \(\ge\)1 | 39.6 | 150 | 6 | Hexagon | 5 | 10 |
