Extended Data Fig. 3: Sequence alignment of humanized nanobodies.
From: An adaptive autoregressive diffusion approach to design active humanized antibodies and nanobodies
a, Sequence alignment of the parental nanobody 3-2A2-4 and its humanized variants. Residues in the complementarity-determining regions (CDRs) are highlighted in green. Residues in light blue correspond to CDR2, as defined by the Llamanade classification. Key residues preserved through the ‘inp’ sampling method are shown in purple. Red underlined residues indicate mutations introduced in the humanized nanobodies relative to the parental nanobody. b, Sequence alignment of the nanobody hC3Nb3 and its corresponding humanized variant. CDR regions are highlighted in green. The last sequence represents the variant generated using the CDR grafting method.
