Extended Data Fig. 4: MTSS1 and ACTBL2 regulate p65 protein.
From: MTSS1 suppresses mammary tumor-initiating cells by enhancing RBCK1-mediated p65 ubiquitination
(a) Transcription factors enriched for the Mtss1-downregulated genes identified by promoter binding site analysis. (n =710 downregulated genes). (b) GSEA analyses of NF-κB target gene sets in TCGA breast tumors with different MTSS1 expression levels. See Online Methods for information of the NF-κB target gene sets. (n = 881 patients). (c) P65 protein level in total cell lysate of preneoplastic mammary glands, the Lin+ cells of the glands and bone marrow cells of Mtss1 WT and KO mice. (d) P65 protein level after MTSS1 overexpression in different breast cancer cells. Numbers are p65 relative levels normalized to loading control. (e) P65 protein level after MTSS1 knockdown in different breast cancer cells.(f) P65 protein level in SCP28 after Mtss1 and Actbl2 overexpression. (g) Expression of various signaling pathway molecules in Py8119 after Mtss1 and Actbl2 overexpression. Statistical significance was determined by Wald test with Benjamini–Hochberg’s multiple-comparison correction (B). The experiments in C-G were repeated three times independently with similar results, and the results of one representative experiment are shown. Unprocessed immunoblots are shown in Source Data Extended Data Fig. 4.
