Extended Data Fig. 4: Loss of autophagy increases high TMB tumor growth in an IFNγ-dependent manner. | Nature Cancer

Extended Data Fig. 4: Loss of autophagy increases high TMB tumor growth in an IFNγ-dependent manner.

From: Autophagy promotes growth of tumors with high mutational burden by inhibiting a T-cell immune response

Extended Data Fig. 4: Loss of autophagy increases high TMB tumor growth in an IFNγ-dependent manner.The alternative text for this image may have been generated using AI.

a, Survival analysis of Atg7+/+ (n = 16 mice), Atg7Δ/Δ (n = 14 mice), IFNγ-/-;Atg7+/+ (n = 6 mice) and IFNγ-/-;Atg7Δ/Δ (n = 5 mice) hosts. b, Representative H&E tissue staining from IFNγ-/-;Atg7+/+ and IFNγ-/-;Atg7Δ/Δ hosts. Images are representative of two independent experiments. c, d, e, f. Comparison of UV YUMM1.1-9 tumor growth on male (c, d) Atg7+/+ (n = 18 tumors), IFNγ-/-;Atg7+/+ (n = 18 tumors), Atg7Δ/Δ (n = 10 tumors), IFNγ-/-;Atg7Δ/Δ (n = 18 tumors) and female (e, f) Atg7+/+ (n = 16 tumors), IFNγ-/-;Atg7+/+ (n = 18 tumors), Atg7Δ/Δ (n = 16 tumors), IFNγ-/-;Atg7Δ/Δ (n = 18 tumors) hosts. Tumor pictures (c, e), and tumor weight and ratio (antibody treated/control) (d, f) from the experimental endpoint. g, Cropped Western blotting showing expression of B2m in MB49 shC and shB2m tumors from Atg7+/+ and Atg7Δ/Δ hosts (n = 3 tumors each) from one experiment. Actin was used a loading control. All data are mean +/- s.e.m. **P < 0.01, ***P < 0.001 using two-sided Student’s t-test. Source data available for d, f.

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