Extended Data Fig. 1: CD1d-specific VHH1D12 activates type I NKT cells. | Nature Cancer

Extended Data Fig. 1: CD1d-specific VHH1D12 activates type I NKT cells.

From: A single-domain bispecific antibody targeting CD1d and the NKT T-cell receptor induces a potent antitumor response

Extended Data Fig. 1: CD1d-specific VHH1D12 activates type I NKT cells.The alternative text for this image may have been generated using AI.

a-f, Expression (%) of CD25 or CD107a on type I NKT cells after 24 h or 4 h co-culture with either HeLa wild type (WT), HeLa.CD1d (a,c,f), MM.1 s.WT, MM.1 s.CD1d cells (b), A431 (d) or CCRF-CEM (e), plus NC, α-GalCer (100 ng/ml), VHH1D control (100 nM or concentration range (e)) and/or VHH1D12 (100 nM or concentration range (e)) (n = 3 (a-d, f) or n = 4 (e) independent experiments with type I NKT cells obtained from individual donors), analysed by flow cytometry. Transwell; type I NKT cells were placed in the upper chamber. g, Representative histogram depicting expression of CD1d (by means of binding of biotinylated VHH1D22) to MM.1S.CD1d after 24 h incubation with negative control (NC) or VHH1D12 (100 nM). h, Exemplifying gating strategy used for type I NKT cell CD107a expression analysis (here after co-culture with CCRF-CEM cells). a-d,f, The bars indicate the mean +s.d.; e, nonlinear regresssion with 95% confidence bands (dotted lines). a,d, One-way analysis of variance (ANOVA) with Tukey multiple comparisons test; b,c, two-way ANOVA with Šídák; f, Tukey multiple comparisons test.

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