Extended Data Fig. 4: Quantitative PCR validation of top ranked genes differentially expressed in CNS blasts compared to spleen in SEM and REH cells. | Nature Cancer

Extended Data Fig. 4: Quantitative PCR validation of top ranked genes differentially expressed in CNS blasts compared to spleen in SEM and REH cells.

From: Metabolic adaptation of acute lymphoblastic leukemia to the central nervous system microenvironment depends on stearoyl-CoA desaturase

Extended Data Fig. 4

a, and 018z cells (b) p (two tailed) = one sample T and Wilcoxon test. Results are normalized to 36B4 human housekeeping genes and presented as LogFold2 change enrichment of comparing CNS to spleen for the SEM-REH samples; human HPRT was used as housekeeping gene and enrichment of comparing CNS to BM was calculated for 018z. n=7 for SCD, FASN, ACLY, CPT1a; n=6 for CPT1b; n=5 for CPT2 in (a). n=12 for LDLR, HMGCR, FASN, ACLY, CPT1a, CPT1b; n=11 for SQLE; n=7 for SCD, ABCA1 in b, For box-and-whisker plots, boxes represent 25th and 75th percentiles, center lines indicate median values and whiskers represent minimum and maximum values. c, Western blot of SCD and FASN proteins in SEM cells retrieved from the CNS and spleen of mice (n=4 mice).

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