Extended Data Fig. 4: CAR-Ts release from hydrogels and chitosan-PEG hydrogel encapsulated GD2.CAR-Ts effectively eliminated tumor.
a, Viability of CAR-Ts released from alginate-gelatin and chitosan-PEG injectable hydrogel (n =3 donor/group). b, CAR-T cell release profile from alginate-gelatin and chitosan-PEG injectable hydrogel (n =3 donors/group). RB tumor cell lines were co-cultured with GD2.CAR-Ts or CD19.CAR-Ts at the effector to tumor ratio of 1:1 (c) and 1:5 (d) for 7 days upon encapsulation with the hydrogels. All cells were collected and analyzed by flow cytometry to enumerate T cells and residual tumor cells in the culture (n =3 donors/group). Data presented show mean and SEM.
