Extended Data Fig. 9: YAP/TAZ are required for GSC maintenance in vitro.
From: Single-cell analyses reveal YAP/TAZ as regulators of stemness and cell plasticity in glioblastoma
a, Control experiment of Fig. 5a–e. Gliomaspheres derived from HER2CA-transformed Yapfl/fl; Tazfl/fl newborn astroglial cells, not expressing CREERT2, were treated with either ethanol (Vehicle) or 4OH-TAM (TAM). Panels are representative images (left; scale bar, 100 µm) and quantifications (right; mean ± s.d. of two independent experiments, each performed with two replicates) of the number of gliomaspheres/cm2 in vehicle versus 4OH-TAM-treated samples. p-values were determined by two-way ANOVA with Sidak’s multiple comparisons test. In the absence of CREERT2 expression, treatment with 4OH-TAM is inconsequential for gliomasphere formation, indicating that gliomasphere disaggregation shown in Fig. 4a–e is specifically caused by YAP/TAZ deletion. b, P2 gliomaspheres derived from R26CAG-CreERT2; Yapfl/fl; Tazfl/fl newborn astroglial cells transformed with the indicated oncogenes were dissociated to single cells and replated at clonal density for P3 gliomasphere formation in presence of ethanol (YAP/TAZ wt), or of 4OH-TAM to induce YAP/TAZ knockout (YAP/TAZ KO). Data are presented as scatter dot plots (n = 3 replicates each) and bar graphs showing mean with s.d. The p-values were calculated by unpaired two-tailed t-test.
