Extended Data Fig. 8: CD8+ T cell mediated killing of bystander OVCAR5 tumor cells.
From: Long-distance modulation of bystander tumor cells by CD8+ T-cell-secreted IFN-γ
A mixture of GFP+ Ag+, CFP+ Ag- IFNγR proficient and CFP+ Ag- IFNγR deficient OVCAR5 cells (2:1:1 ratio) was treated with CDK4R>L-specific CD8+ T cells at a 2:1 T cell: tumor cell ratio, or left untreated, and cell survival was analyzed by staining with IR-Dye and subsequent flow cytometry. a, Representative plots depicting the percentage of IR-Dye+ cells for the indicated groups. b, Quantification of a, bar graph shows mean of n=3 technical replicates. Representative data of two independent experiments are depicted.
