Extended Data Fig. 3: Impact of genetic loss of Stau2 on normal HSC function.
a, Relative Stau2 expression in whole bone marrow cells isolated from Stau2+/+ and Stau2−/− mice (n = 3 technical replicates per group). b, Total number of cells (mean±S.E.M.) in the bone marrow of Stau2+/+ and Stau2−/− mice (n = 4 animals per cohort; data combined from 3 independent experiments; two-tailed Student’s t-test). c-d, Frequency of committed progenitors (c) and differentiated cells (d) in the bone marrow of Stau2+/+ and Stau2−/− mice (mean±S.E.M.; n = 4 animals per cohort; data combined from 3 independent experiments; two-tailed Student’s t-test). e, Frequency of donor-derived Stau2+/+ or Stau2−/− cells in the bone marrow of recipient mice transplanted with 500 HSCs of each genotype, 4 months post-transplant (mean±S.D.; n = 4 animals per cohort; data combined from 3 independent experiments). f-g, Frequency of indicated donor-derived hematopoietic stem and progenitor and differentiated cell populations in the bone marrow of recipient mice transplanted with Stau2+/+ or Stau2−/− HSCs 4 months post-transplant (mean±S.D.; n = 4 animals per cohort; data combined from 3 independent experiments). h, Frequency of donor-derived Stau2+/+ or Stau2−/− cells in the bone marrow of secondary transplant recipients, 4 months post-transplant (mean±S.E.M; n = 4 animals per cohort; data combined from 3 independent experiments). i-j, Frequency of indicated donor-derived hematopoietic stem and progenitor and differentiated cell populations in the bone marrow of secondary transplant recipients, 4 months post-transplant (mean±S.D.; n = 4 animals per cohort; data combined from 3 independent experiments). k, Relative Stau1 expression in normal hematopoietic cells from Stau2+/+ and Stau2−/− mice (left panel) or in Lin- leukemia cells (right panel).
