Extended Data Fig. 10: Bootstrap analysis for age-association assessment within IntClust subgroups.

A random sample with replacement of size N was taken from the METABRIC expression set (1992 cases in total) in each of the ten IntClust groups to yield equally sized IntClust groups, so as to avoid the issue of smaller p-values in larger IntClust groups as seen in Fig. 4 where IntClust groups 3, 4 and 8 appear to show more age-correlated gene targets. Age-association status was reassessed for all gene targets with the bootstrapped sample and the proportion of age-correlated targets within each IntClust group recalculated, as shown by the red points plotted. Proportion of age-associated genes from each of the 20 bootstrap analyses are shown along with their associated boxplot, suggesting that IntClust groups 1, 2, 6 and 9 also contain sizable proportions of gene targets with age-correlated expression. The bootstrap sample size N per IntClust group is shown at the top of each graph. Boxplot elements show median (centre line), first and third quartiles (box top and bottom “hinges”), and whiskers extending from the hinge to the largest value no further than 1.5 * IQR from the hinge (where IQR is the inter-quartile range, or distance between the first and third quartiles).