Extended Data Fig. 6: Characterization of T cell phenotype in total and dendritic cell (DC) PD-L1 deficient mice.
From: PD-L1 expression by dendritic cells is a key regulator of T-cell immunity in cancer
a, Frequencies and b, numbers of total (left) and subsets (right) of splenic CD11c + MHCII + cells from control Cd274fl/fl and Clec9a.Cre Cd274fl/fl mice. Circles represent individual mice (n = 5 per group), solid bars represent mean values, and error bars represent the mean ± standard deviation. Pooled data from two experiments. Statistics were calculated using the two-tailed, unpaired Student’s t-test with Welch’s correction. c, Frequencies of CD62L + CD44- CD8 + T cells in lymph nodes and spleens. d-e, Frequencies of CD62L- and CD44-expressing subsets of CD8 + T cells in d, lymph nodes and e, spleens. f, Frequencies of CD62L + CD44- CD4 + Foxp3- T cells in lymph nodes and spleens. g-h, Frequencies of CD62L- and CD44-expressing subsets of CD4 + Foxp3- T cells in g, lymph nodes and h, spleens. i, Frequencies of Foxp3-expressing CD4 + T cells in lymph nodes and spleens. j-k, Frequencies of CD62L- and CD44-expressing subsets of CD4 + Foxp3 + T cells in j, lymph nodes and k, spleens. Circles represent individual mice (Control and total PD-L1 knockout n = 3, PD-L1ΔDC n = 2), bars represent mean values, and error bars represent the mean ± standard deviation. Data are from a single experiment.
