Extended Data Fig. 9: Dendritic cell-specific loss of PD-L1 results in enhanced T cell activation.
From: PD-L1 expression by dendritic cells is a key regulator of T-cell immunity in cancer
a, Frequency of MC38 neoantigen-specific CD8 + T cells in the draining lymph nodes of tumor-bearing mice at day 10 post-tumor inoculation. b, ELISPOT analysis of IFN-γ production by draining lymph node cells following stimulation with neoantigen peptides. Analysis is at day 10 post-tumor inoculation. c, Frequency of MC38 neoantigen-specific CD8 + T cells in tumors at day 10 post-tumor inoculation. a-c, Circles represent individual mice (Control and PD-L1ΔDC n = 10, total PD-L1 knockout n = 8), bars represent mean values, and error bars represent the mean ± SEM. Statistics were calculated using the Mann-Whitney test. ns = not significant. Data is from a single experiment. d, Representative flow cytometry plots of H2-Kb SIINFEKL Dextramer staining on splenic CD8 + T cells on day 7 following immunization with DEC-Ova and anti-CD40 antibody. e, Frequencies of SIINFEKL Dextramer positive cells among CD8 + T cells. n = number of mice (non-immunized n = 5, control n = 10, total PD-L1 knockout n = 9, PD-L1ΔDC n = 8). Statistics were calculated using the Mann-Whitney test. Pooled data from two experiments. f, Representative flow cytometry plots of CD62L and CD44 expression among SIINFEKL Dextramer-specific CD8 + T cells. g, Frequencies of CD62L-expressing (left) and CD44 + CD62L- (right) cells among SIINFEKL Dextramer-specific CD8 + T cells. h, Representative histograms of PD-1 expression on SIINFEKL Dextramer-specific CD8 + T cells. i, Frequencies of PD-1-expressing cells among SIINFEKL Dextramer-specific CD8 + T cells. j, Mean fluorescence intensity (MFI) of PD-1 expression on SIINFEKL Dextramer-specific CD8 + T cells. d-j, Data are representative of three independent experiments g, i-j, Circles represent individual mice (control n = 5, total PD-L1 knockout and PD-L1ΔDC n = 6) bars represent mean values, and error bars represent the mean ± standard deviation. Statistics were calculated using the two-tailed, unpaired Student’s t-test with Welch’s correction. * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001.
