Extended Data Fig. 8: Gating strategy for flow cytometry analysis of dendritic cells and pmel T cells. | Nature Cancer

Extended Data Fig. 8: Gating strategy for flow cytometry analysis of dendritic cells and pmel T cells.

From: Multifunctional oncolytic nanoparticles deliver self-replicating IL-12 RNA to eliminate established tumors and prime systemic immunity

Extended Data Fig. 8

C57Bl/6 mice bearing established B16F10 tumors and TDLNs were necropsied and prepared as single cells suspension. The cells were stained with antibodies against cell surface makers as indicated and followed with flow cytometry (see methods). a. Shown are gating strategies of conventional DC1 and DC2 cells in TDLNs for Fig. 6f. b. Shown are gating strategies of Pmel CD8 T cell activation by cells from inguinal lymph nodes for Fig. 6g.

Back to article page