Extended Data Fig. 3: Resident memory CD8+ T cells expressing CD69 reside in both the epidermis and dermis in long-term metastatic melanoma survivors with immunotherapy-associated vitiligo. | Nature Cancer

Extended Data Fig. 3: Resident memory CD8+ T cells expressing CD69 reside in both the epidermis and dermis in long-term metastatic melanoma survivors with immunotherapy-associated vitiligo.

From: Resident and circulating memory T cells persist for years in melanoma patients with durable responses to immunotherapy

Extended Data Fig. 3: Resident memory CD8+ T cells expressing CD69 reside in both the epidermis and dermis in long-term metastatic melanoma survivors with immunotherapy-associated vitiligo.

a, Representative pseudocolor dot plots showing the gating strategy for the live CD45+ CD3+ CD8+ population. b, Contour plots show the expression of CD69, CD103 and CD62L on pre-gated CD8+ population by flow cytometry. The number represents the proportion out of total CD8+ T cells. c, Immunohistochemistry staining for CD8+ CD69+ resident memory CD8+ T cells in the skin from patient PT628. Images are representative of multiple fields from at least three skin sections taken from each of 4 individual patients. CD8 was stained in green and CD69 was stained in red. CD8+ and CD69+ single-stain cells are indicated by green and red arrows, respectively. Black cells (indicated by black arrows) are CD8+CD69+ co-stained cells. Original magnification: 10X (left) and 40X (right).

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