Extended Data Fig. 10: Lipid availability determines response to pharmacological FASN inhibition.
From: Fatty acid synthesis is required for breast cancer brain metastasis
a-c, BT474 brain tumor-derived organotypic slice cultures were treated for 6 d with vehicle or 500 nM TVB3166 in standard (+Lipids) or delipidated (-Lipids) media. The percent of apoptotic and viable HER2 positive BT474 cells was determined by measuring Annexin V (AV) and 7-amino actinomycin D (7AAD) uptake by flow cytometry. The flow cytometry gating strategy is presented in Supplementary Fig. 2. *P = 0.0111, ***P = 0.0003 by one-way ANOVA followed by Tukey´s test (n = 5 BT474 brain tumor slices). d,e, Mouse weights for the cohort presented in Fig. 6f,g that was treated daily with BI99179 (15 mg/kg) or vehicle control by oral gavage (n = 5 mice). Data presented in all panels are means ± s.e.m.
