Extended Data Fig. 3: Higher lipid synthesis in breast cancer lesions when compared to surrounding brain tissue.
From: Fatty acid synthesis is required for breast cancer brain metastasis
a, Negative mode matrix assisted laser desorption/ionization–mass spectrometry imaging (MALDI-MSI) of brain tissue from a separate NSG mouse bearing a BT474 brain tumor than that shown in Fig. 1f,g that had been given 4 daily bolus intraperitoneal injections of 2 g/kg 13C-glucose. The spatial distribution of the indicated isotopologues of palmitate, stearate, oleate, and lysophosphatidylinositol (Lyso PI, 18:0) normalized to total ion counts are shown. b,c, HER2 immunohistochemistry (IHC) staining of brain tissue sections collected from the tumors analyzed by MALDI-MSI in Fig. 1g (b) and in Extended Data Fig. 3a (c). d, Negative mode MALDI-MSI of the m + 2 palmitate isotopologue presented in a normalized to m + 0 palmitate. For all panels, scale bar = 1 mm.
