Extended Data Fig. 3: Immunohistochemistry (IHC) analysis of immune cells.
Immunohistochemistry (IHC) analysis of immune cells. a, Each tissue section was manually annotated in image analysis software HALO™ into non-tumor and tumor regions (live, necrosis). b, Density of CD3, CD8, and CD20 in post-treatment surgical samples in the non-tumor regions by IHC (nonresponder, n = 4 patients; responder, n = 3 patients) (mean ± s.d., all P > 0.05, unpaired two-tailed t-test). c, Representative staining results of baseline core biopsies (PRE) and post-treatment surgical samples (POST) for CD3, CD8, and CD20 IHC (one patient selected from each group, representative of n = 3 nonresponder, n = 2 responder paired patient samples; quantitative data shown in panel b).
