Extended Data Fig. 2: ß-diversity analysis of the mouse feces after the fecal transplantation from the human subjects.
From: Chronic liver disease enables gut Enterococcus faecalis colonization to promote liver carcinogenesis
(a) Microbiota-depleted mice received the fecal microbiota from the subjects HD2, CLD1, CLD6, HCC8 or HCC14. Unweighted UniFrac distances were calculated with taxonomy data in feces of the mice at various time points after the fecal transplantation. The results are visualized by principal coordinate analysis. d, day; M, months. (b) Chao1 was calculated from taxonomy data. Boxes represent 25th-75th percentile of the distribution. The median is shown in the middle of the boxes. Whiskers represent min to max. The number of samples were 21–24 and 10 iterations were performed in the analysis. (c to f) Germ-free or antibiotics-pretreated mice received feces of thee subjects HD50, CLD9 and HCC11 (c), HD55, CLD13 and HCC12 (d), and HD59, CLD10 and HCC13 (e). Unweighted UniFrac distances were calculated with taxonomy data in feces of the mice and visualized. (f) UniFrac distances between the subjects and feces-transplanted germ-free mice, and between those and feces-transplanted antibiotics-pretreated mice were compared. ABX, antibiotics; FT, feces-transplanted. n=9. (b), Kruskal Wallis test; (f), two-sided Mann-Whitney U test. *, P < 0.05; ***, P < 0.001. Exact P-values were as follows: (b) HD vs. CLD, P<0.0001; HD vs. HCC, P<0.0001; CLD vs. HCC, P=0.0139: (f) P=0.0244.
