Extended Data Fig. 4: Liver gene expression after transplantation of the subjects’ feces to germ-free mice.
From: Chronic liver disease enables gut Enterococcus faecalis colonization to promote liver carcinogenesis
Germ-free mice received fecal transplantation from the selected subjects, HD50, CLD9 and HCC11 (a and d), HD55, CLD13, HCC12 (b), HD59, CLD10, and HCC13 (c). (a–c) Then, after DEN injection once followed by CCl4 injection twice, the mice were euthanized and mRNA expression in the liver was analyzed by real-time PCR. n=4–5. (d) The mice received DEN injection twice followed by CCl4 injection and were euthanized in 8 months after the DEN injection. n=5. Means and SE are shown. GF, germ-free. (a–c), one-way ANOVA followed by Tukey’s test; (d), Kruskal-Wallis test. *, P < 0.05; **, P < 0.01; ***, P < 0.001. Exact P-values were as follows: (a) mki67 HD50 vs. CLD9, P=0.0073; HD50 vs. HCC11, P=0.0072; HD50 vs. GF, P=0.998; CLD9 vs. HCC11, P>0.999; CLD9 vs.GF, P=0.0054; HCC11 vs. GF, P=0.0053: ccnb1 HD50 vs. CLD9, P=0.0314; HD50 vs. HCC11, P=0.0209; HD50 vs. GF, P=0.999; CLD9 vs. HCC11, P=0.996; CLD9 vs.GF, P=0.0378; HCC11 vs. GF, P=0.0252: col1a1 HD50 vs. CLD9, P=0.433; HD50 vs. HCC11, P=0.181; HD50 vs. GF, P=0.303; CLD9 vs. HCC11, P=0.932; CLD9 vs.GF, P=0.0186; HCC11 vs. GF, P=0.0056: tnf HD50 vs. CLD9, P=0.151; HD50 vs. HCC11, P=0.0219; HD50 vs. GF, P=0.0432; CLD9 vs. HCC11, P=0.736; CLD9 vs.GF, P=0.0005; HCC11 vs. GF, P<0.0001: birc5 HD50 vs. CLD9, P=0.0053; HD50 vs. HCC11, P=0.010; HD50 vs. GF, P=0.998; CLD9 vs. HCC11, P=0.989; CLD9 vs.GF, P=0.0040; HCC11 vs. GF, P=0.0075: (b) mki67 HD55 vs. CLD13, P=0.0011; HD55 vs. HCC12, P=0.0003; CLD13 vs. HCC12, P=0.507: ccnb1 HD55 vs. CLD13, P=0.0031; HD55 vs. HCC12, P<0.0001; CLD13 vs. HCC12, P=0.045: col1a1 HD55 vs. CLD13, P=0.146; HD55 vs. HCC12, P=0.248; CLD13 vs. HCC12, P=0.929: tnf HD55 vs. CLD13, P=0.122; HD55 vs. HCC12, P=0.757; CLD13 vs. HCC12, P=0.339: birc5 HD55 vs. CLD13, P=0.0174; HD55 vs. HCC12, P<0.0001; CLD13 vs. HCC12, P=0.0047: (c) mki67 HD59 vs. CLD10, P=0.455; HD59 vs. HCC13, P=0.0205; CLD10 vs. HCC13, P=0.172: ccnb1 HD59 vs. CLD10, P=0.692; HD59 vs. HCC13, P=0.100; CLD10 vs. HCC13, P=0.354: col1a1 HD59 vs. CLD10, P=0.305; HD59 vs. HCC13, P=0.177; CLD10 vs. HCC13, P=0.929: tnf HD59 vs. CLD10, P=0.365; HD59 vs. HCC13, P=0.0403; CLD10 vs. HCC13, P=0.383: birc5 HD59 vs. CLD10, P=0.383; HD59 vs. HCC13, P=0.0467; CLD10 vs. HCC13, P=0.405: (d) HD50 vs. CLD9, P=0.303; HD50 vs. HCC11, P=0.019; CLD9 vs. HCC11, P=0.839.
