Extended Data Fig. 8: Effect of E. faecalis is not based on initiation of carcinogenesis by DNA damage.
From: Chronic liver disease enables gut Enterococcus faecalis colonization to promote liver carcinogenesis
(a, b) After transplantation of feces of HD, CLD, or HCC, or inoculation of E. faecalis strain V583, DEN was injected. One day later liver was harvested and stained with anti-phospho-H2AX antibody. (a) Representative pictures of phospho-H2AX staining from 2 independent experiments are shown. Bars, 50µm. (b) Percentages of phospho-H2AX-positive nuclei were compared between HD, CLD and HCC, or V583 WT and gelE-deleted V583. n=24 or 12, respectively. (c) After inoculation of V583, CCl4 was injected with or without DEN, and 8 months later number of liver tumors was counted. Means and SE are shown. n=12. (b) Kruskal-Wallis test for 3 groups and two-sided Mann-Whitney U test for 2 groups. (c) two-sided Mann-Whitney U test. ***, P < 0.001. Exact P-values were as follows: (b) HD vs. CLD, P>0.999; HD vs. HCC, P>0.999; CLD vs. HCC, P>0.999: V583WT vs. V583ΔgelE, P=0.932: (c) P<0.0001.
